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当前位置:欢乐生肖 > 抗原抗体、ELISA、WB > 一抗 > Antibodies against Ca2+ -bindin > Monoclonal anti Calbindin D-28k
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Monoclonal anti Calbindin D-28k

300-1 ml
货号 货期 规格 / 价格 询价

Monoclonal anti Calbindin D-28k

品牌

Swant

货号

300-1 ml

产品分类

Antibodies against Ca2+ -bindin

研究领域

Calbindin

产品概述

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

形式

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. McAB 300 reacts specifically with calbindin D-28k on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (but probably not fish, Fig. 1). McAB 300 does not cross-react with calretinin or other known calcium binding-proteins. McAB 300 specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000, IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 1012 L/M. McAB 300 against calbindin D-28k specifically localizes calbindin using free-floating or mounted sections of brain, kidney and pancreas of probably all vertebrates with the exception of fishes.

浓度

Calbindin D-28k

纯度

Mouse monoclonal, IgG1

试剂准备

Lyophilized concentrated supernatant (no preservatives).

复溶

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

应用

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Lot No: 07 (F) Reconstitution: with 200 µl / 500 µl or 1 ml bidistilled water. Immunoblot of brain extracts: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected. Immunohistochemistry on Calbindin D-28k knock-out mice: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected. Product: Conc. supernat.

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