您好,欢迎光临安诺伦(北京)生物科技商城!
全国服务热线:400-965-8633
关注我们
  • 会员中心
  • 会员中心
    询价列表
    0

    最新加入的商品

    0 件商品 合计:¥ 0
  • 会员中心
    购物车

    最新加入的商品

    件商品 合计:¥ 0

全部产品分类

自主品牌
当前位置:重庆时时彩 > 抗原抗体、ELISA、WB > 一抗 > Antibodies against Ca2+ -bindin > Monoclonal anti parvalbumin
品牌:
  • 所有品牌
  • A
  • B
  • C
  • D
  • E
  • F
  • G
  • H
  • I
  • J
  • K
  • L
  • M
  • N
  • O
  • P
  • Q
  • R
  • S
  • T
  • U
  • V
  • W
  • X
  • Z

Monoclonal anti parvalbumin

235-1 ml
货号 货期 规格 / 价格 询价
Images

Monoclonal anti parvalbumin

品牌

Swant

货号

235-1 ml

产品分类

Antibodies against Ca2+ -bindin

研究领域

Parvalbumin

产品概述

For long storage, keep small aliquots of 1-2 µl at - 80°C (or at least - 20°C). For continous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

形式

Monoclonal anti-Parvalbumin is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with parvalbumin purified from carp muscles (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labeling as well as Avidin-Biotin staining of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay (RIA)(1). The product is a monoclonal antibody (McAB) against parvalbumin, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. The antibody reacts specifically with parvalbumin in tissue originating from human, monkey, rabbit, rat, mouse chicken and fish. The McAB specifically stains the 45Ca-binding spot of parvalbumin (MW 12'000 and IEF 4.9) in a two-dimensional "immunoblot". In a RIA set up the McAB measures parvalbumin with a sensitivity of 10 ng/assay and an affinity of 7.9 x 1012 L/M (1).

浓度

Parvalbumin

纯度

Mouse monoclonal, IgG1

试剂准备

Lyophilized ascites (no preservatives).

复溶

1. Celio M.R. et al., Cell Calcium 9:81-86, 1988 2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302 3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506 4. Celio M.R. (1986) Science 231:995-997 5. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403

应用

Immunohistochemistry: 1:5'000 - 1:10'000 Immunoblotting: 1:1'000. We recommend that the optimal dilution be determined by titration test.

产品描述

Lot No: 10-11 (F) Reconstitution: with 200 µl bidistilled water. Immunoblot: The specificity of antibody 235 could be determined by immunoblots of brain and muscle extracts (1). However, this antibody does not recognize well the parvalbumin molecule after SDS-gel electrophoresis. Immunohistochemistry on Calbindin D-28k knock-out mice: Antibody 235 immunolabels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of parvalbumin knock out mice (Fig. 2b). Product: Conc. supernat. uses: Calcium binding-proteins represent a family of small, acidic proteins equipped with peculiar cavities which accept Ca2+ with high selectivity. There are two types of calcium binding-proteins, “trigger” and “buffer” proteins. Those of the “trigger”-type (e.g. calmodulin and troponin-C) act by changing shape upon binding Ca2+. This distortion exposes regions on the surface of the protein, which interact with surrounding target molecules, altering their activity. The calcium binding-protein of the “buffer”-type are conceived as a system which is in charge of controlling the Ca2+ concentration inside cells. Parvalbumin occurs mainly in subpopulations of nerve cells (2,4) and in fast muscle fibers (3). It might confer on these cells peculiar skills in the handling of calcium-ions. Parvalbumin is an attractive neuroanatomical tool and this McAB guarantees the Calcium binding-proteins represent a family of small, acidic proteins equipped with peculiar cavities which accept Ca2+ with high selectivity. There are two types of calcium binding-proteins, “trigger” and “buffer” proteins. Those of the “trigger”-type (e.g. calmodulin and troponin-C) act by changing shape upon binding Ca2+. This distortion exposes regions on the surface of the protein, which interact with surrounding target molecules, altering their activity. The calcium binding-protein of the “buffer”-type are conceived as a system which is in charge of controlling the Ca2+ concentration inside cells. Parvalbumin occurs mainly in subpopulations of nerve cells (2,4) and in fast muscle fibers (3). It might confer on these cells peculiar skills in the handling of calcium-ions. Parvalbumin is an attractive neuroanatomical tool and this McAB guarantees the Antibody performance: McAB 235 against parvalbumin specifically localizes parvalbumin using free-floating or mounted sections of brain and muscles probably of all vertebrates. Fixation does not seem to be an hindrance to the detection of the antigen since even 2.5% glutaraldehyde was compatible with immunolocalization on semithin (0.5 µm thin) cryo-sections of the rat cortex (4). It can be used with rat-parvalbumin as a tracer to quantify parvalbumin in the brain of various species (1).

图像

Specification

官网链接

https://www.swant.com/pdfs/Monoclonal_parvalbumin_235.pdf

数量

Related Products

Select Brand Catalog No. Product Name Pack Size Type Field of Research Specification Quantity Price(USD)

This item has successfully been added to your cart!

You have 0 item in your cart

Your inquiry has successfully been added to your list!

You have 0 item in your inquiry list

快3在线投注 重庆时时彩玩法 极速快乐8 极速11选5 重庆时时彩计划 重庆时时彩计划 重庆时时彩计划 极速赛车 快三投注平台 重庆时时彩网上投注